screened positive meaning in Chinese
加网阳片
Examples
- We screened the cdna expression library by using 12 b - deoxoartemisinyl - ( 4 ' - oxyacetic acid ) phenyl ether conjugated with bsa . one of the screened positive clones was inserted tctp of plasmodium falciparum from hainan . the tctp was cloned to express protein in order to provide materials for further study
把青篙素类衍生物: 12b一二氢青篙素( 4 ’一氧乙酸基)苯醚用bsa标记作为探针，对恶性疟原虫海南株cdna表达文库筛选，分离出插入了恶性疟原虫海南株tctp基因的阳性单克隆。 - The e2 gene was amplified by rt - pcr , then examined the fragment by electrophoresis . after purification and insertion into pucm - t vecter , the recombinant plasmid pbne2pi and pbne2pii were obtained . then they were transfected e . coli jm109 and screened positive clones by blue or white plaques . the recombinant plasmids were extracted and the inserted fragments were identificated by electrophoresis
通过rt - pcr扩增e _ 2基因，电泳测定扩增片段的大小，经纯化后，连接于pucm - t载体，获得重组质粒pbne2p 、 pbne2p ，转化e ? colijm109 ，经蓝白斑筛选，挑取阳性克隆，提取质粒，直接电泳鉴定和酶切鉴定。